Innovative technologies for
biomolecular interaction analysis
Pharma Diagnostics’ vision is to transform biomolecular interaction analysis – we are the first company to enable SPR label-free analysis of protein-protein interactions on a standard absorbance plate reader.
Our SoPRano™ technology is unique in that no specialized SPR instrument is required. SoPRano™ uses a variant of SPR known as LSPR (Localised Surface Plasmon Resonance) whereby gold nanomaterials conjugated to a biomolecule exhibit specific changes in absorbance upon ligand interaction which can be read on a standard absorbance plate-reader.
SoPRano™ Gold Nano Rod (GNR) technology enables high throughput, plate-based, homogenous SPR assays. SoPRano™ delivers both the affinity of interaction (KD) and kinetics, ka (association rate or on-rate) and kd (dissociation rate or off-rate), as well as standard end-point assays. Furthermore, the long read-times possible with SoPRano™ assays (currently up to ~ 1 hour) enable data to be collected to equilibrium for systems with slower association (on-rates, ka) or dissociation (off-rates, kd).
SoPRano™ is available in kit form, and because it only requires a standard absorbance plate reader, delivers kinetics data at a fraction of the investment cost of current SPR methods which require an expensive, specialized instrument. This enables the broader application of SPR label-free biomolecular interaction analysis by reducing the financial barrier of adoption.
SoPRano™ is highly flexible, with diverse applications in basic research and therapeutic R&D, including:
- Biomolecular interaction kinetics
- Antibody screening
- Protein-protein interactions analysis
- Protein-protein small molecule inhibition assays.