Applications

SoPRano™ is broadly enabling and has been adapted for a variety of applications in basic research and pharmaceutical discovery and QC.

For information on SoPRano GNR characteristics, QC and stability please click here

Antibody Screening

Detection of the interaction of anti-human Serum Albumin (hSA)  monoclonal antibody (mAb) (Abcam, #ab18081) on hSA conjugated to GNRs (hSA-GNR).

Assay conditions: 384-well plate Total volume 80µL n = 3 S/N = 24.1 Z’-factor = 0.83 Incubation time = 35 min	Read on BMG SPECTROstar Omega. λmax was determined to be 766nm. Ratio OD766nm+80nm /OD766nm was calculated and data were analyzed with one site total binding model (GraphPad Prism software). Experiment 11FHERR052

The signal for the interaction of anti-hSA mAb with hSA-GNR is specific, as illustrated by the the lack of cross-reactivity of anti-bSA mAb with hSA-GNR.

Assay conditions: 384-well plate Total volume 40µL n = 3 S/N = 22.8 Z’-factor = 0.72 Incubation time = 50 min	Read on BMG SPECTROstar Omega. λmax was determined to be 770nm. Ratio OD770nm+80nm /OD770nm was calculated and data were analyzed with one site total binding model (GraphPad Prism software). Experiment 11FVELD005

Protein-Protein Interaction Inhibitors

Data coming soon.

Protein-Protein Interactions

Data coming soon.