Antibody / Antigen interactions

Pharma Diagnostics’ vision is to transform biomolecular interaction analysis – we are the first company to enable SPR label-free analysis on standard equipment that you already have in your laboratory!

Our SoPRano™ platform enables high throughput, plate-based, homogenous SPR assays using a standard absorbance plate reader, enabling the broader adoption of SPR label-free biomolecular analysis at all stages of basic research and discovery.

We provide services to academic institutions and companies involved in basic protein and antibody research, and the discovery of both small molecule and protein therapeutics.

The SoPRano™ platform is highly flexible, with diverse applications in basic research and therapeutic R&D, including:

  • Antibody screening
  • Protein-protein interactions analysis
  • Protein-protein small molecule inhibition assays.

Antibody Screening

Use SoPRano™ technology to run label-free antibody-antigen interaction screens in high throughput formats without the need for specialist equipment.

 

Applications

The SoPRano™ platform is broadly enabling and has been adapted for a variety of applications in basic research and pharmaceutical discovery and QC.

For information on SoPRano GNR characteristics, QC and stability please click here

Antibody Screening

Detection of the interaction of anti-human Serum Albumin (hSA) monoclonal antibody (mAb) (Abcam, #ab18081) on hSA conjugated to GNRs (hSA-GNR).

Detection of the interaction of anti-human Serum Albumin (hSA) monoclonal antibody (mAb) (Abcam, #ab18081) on hSA-GNR
Assay conditions:
  • 384-well plate
  • Total volume 80µL
  • n = 3
  • S/N = 24.1
  • Z’-factor = 0.83
  • Incubation time = 35 min
Read on BMG SPECTROstar Omega. λmax was determined to be 766nm. Ratio OD766nm+80nm /OD766nm was calculated and data were analyzed with one site total binding model (GraphPad Prism software).

Experiment 11FHERR052

The signal for the interaction of anti-hSA mAb with hSA-GNR is specific, as illustrated by the the lack of cross-reactivity of anti-bSA mAb with hSA-GNR.

Cross Reactivity
Assay conditions:
  • 384-well plate
  • Total volume 40µL
  • n = 3
  • S/N = 22.8
  • Z’-factor = 0.72
  • Incubation time = 50 min
Read on BMG SPECTROstar Omega. λmax was determined to be 770nm. Ratio OD770nm+80nm /OD770nm was calculated and data were analyzed with one site total binding model (GraphPad Prism software).

Experiment 11FVELD005

scientist at work

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